Journal: Nature medicine

Article Title: Identification of small molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

doi: 10.1038/nm.4184

Figure Lengend Snippet: Identification of PHA-690509 and Niclosamide as antiviral compounds. (a) Top, example western blot images of titration of PHA-690509 on SNB-19 cells for anti-ZIKV activity. Glioblastoma SNB-19 cells were treated with the indicated compound for 1 hour prior to inoculation with ZIKV FSS-13025, PRVABC59, or MR766 (MOI = 1) and cells were harvested 24 hours post infection for western blot analysis. Bottom, quantification of NS1/GAPDH protein band intensities. Data were normalized to that with the DMSO treatment. Values represent mean ± s.d. (n = 3 cultures; ***P < 0.001; One-way ANOVA for comparison with the DMSO treatment). Insert shows chemical structure of PHA-690509. (b) Top, example western blot images of titration of Emricasan on SNB-19 cells for anti-ZIKV activity. Similar to (a). Values represent mean ± s.d. (n = 3 cultures; P > 0.1; One-way ANOVA for comparison with the DMSO treatment). (c) Top, chemical structure of Niclosamide and example western blot images of titration of Niclosamide on SNB-19 cells for anti-ZIKV activity using the PRVABC59 strain (MOI = 1). Bottom, quantification similar to (a). Values represent mean ±s.d. (n = 3 cultures; **P < 0.01; ***P < 0.001; One-way ANOVA for comparison with the DMSO treatment). (d) Summary of ELISA quantifications of secreted ZIKV-NS1 protein in the medium of infected cells upon treatment of different doses of Niclosamide (top) or PHA-690509 (bottom) for 24 hours. Values represent mean ± s.d. (n = 3 cultures; ***P < 0.001; One-way ANOVA for comparison with the 0 μM group). (e) Summary of titration of Niclosamide and PHA-690509 on ZIKV viral production. SNB-19 cells were treated with each indicated compound at increasing concentrations for 1 hour prior to infection with PRVABC59 at MOI = 0.5. Cell culture supernatant was collected 24 hours post infection and infectious virions were quantified for focus-forming units (FFU) using Vero cells. Data represent mean ± s.d. (n = 3 cultures). Curves represent best fits for calculating IC50, and the insets report the calculated IC50 value for each compound.

Article Snippet: The anti-ZIKV NS1 ELISA kit (ZKV-NS1-EK) was obtained from BioFront Technologies (Tallahassee, FL) and used according to the manufacturer’s protocol.

Techniques: Western Blot, Titration, Activity Assay, Infection, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Nature medicine

Article Title: Identification of small molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

doi: 10.1038/nm.4184

Figure Lengend Snippet: Inhibition of ZIKV infection by Niclosamide and PHA-690509 at a post-entry step and blockade of ZIKV infection by additional CDKis. (a) Schematic illustration of time-of-addition experiment for Niclosamide and PHA-690509. (b) Top, example western blot images of SNB-19 cells treated with 10 μg/ml anti-AXL antibody, 2 μM Niclosamide, or 92 μM PHA-690509 for 1 hour prior to or 4 hours post infection with PRVABC59 (MOI = 1). Bottom, quantification of NS1/GAPDH protein band intensities. Data were normalized to that with the DMSO treatment. Values represent mean ± s.d. (n = 3 cultures; *P < 0.05; **P < 0.01; ***P < 0.001; One-way ANOVA for comparison with the DMSO treatment). (c) Inhibition of ZIKV infection in CDKi-treated cells. Left, example western blot images of SNB-19 cells treated with the indicated compound at 92 μM for one hour prior to infection with FSS13025 or PRVABC59 (MOI = 1). Right, quantification similar to (b). Values represent mean ± s.d. (n = 3 cultures; ***P < 0.01; One-way ANOVA for comparison with the DMSO treatment). (d) Effect of various CDKis on ZIKV production. Similar to Figure 2e. All data were normalized to 0 μM for each compound. Data represent mean ± s.d. (n = 3 cultures). Curves represent best fits for calculating IC50 (listed on the right panel).

Article Snippet: The anti-ZIKV NS1 ELISA kit (ZKV-NS1-EK) was obtained from BioFront Technologies (Tallahassee, FL) and used according to the manufacturer’s protocol.

Techniques: Inhibition, Infection, Western Blot

Journal: Nature medicine

Article Title: Identification of small molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

doi: 10.1038/nm.4184

Figure Lengend Snippet: Niclosamide and PHA-690509 inhibit ZIKV infection in human astrocytes and forebrain-specific hNPCs. (a) Top, example immunostaining images of astrocytes treated with 2 μM Niclosamide, 92 μM PHA-690509, 9 μM Emricasan, or a combination of 92 μM PHA-690509 and 9 μM Emricasan for 1 hour prior to infection with PRVABC59 (MOI = 0.5). Cells were fixed 24 hours post infection and stained for ZIKVE (green) and DAPI (blue; Scale bar: 50 μm, applies to all image panels in a). Bottom, quantification of the percentage of ZIKV-infected astrocytes over DAPI. Values represent mean ± s.d. (n = 6 cultures; ***P < 0.001; One-way ANOVA for comparison with the DMSO treatment). (b) Top, example western blot images of hNPCs infected at a MOI of 0.1 and analyzed 48 hours post infection. Bottom, quantifications of NS1/GAPDH protein band intensities. Data were normalized to that with the DMSO treatment. Values represent mean ± s.d. (n = 3 cultures; ***P < 0.01; One-way ANOVA for comparison with the DMSO treatment). (c) Virus production from compound treated iPSC-derived human astrocytes. Similar to Figure 2e. Data represent mean ± s.d. (n = 3 cultures). (d–e) Summary of additive effects of a combination of Emricasan and PHA-690509 on inhibiting increased caspase-3 activity in human astrocytes infected with FSS13025-ZIKV or MR766 ZIKV (d), and on improving cell viability as measured by ATP production (e). Similar to Figure 1a. Values represent mean ± s.d. (n = 3 cultures; *P < 0.05; **P < 0.01; One-way ANOVA).

Article Snippet: The anti-ZIKV NS1 ELISA kit (ZKV-NS1-EK) was obtained from BioFront Technologies (Tallahassee, FL) and used according to the manufacturer’s protocol.

Techniques: Infection, Immunostaining, Staining, Western Blot, Derivative Assay, Activity Assay

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Competitive ELISA using synthetic peptides. The recombinant DENV-1 NS1 proteins (20 μg/mL) were coated on an ELISA plate. Aliquots (2 μg/well) of each antibody (Membrane-1, Membrane-2, AuNPs-1 and AuNPs-2) were mixed with each peptide (500 μg/well) corresponding to the amino acid positions shown in the left panel. Peroxidase-conjugated anti-mouse IgG was used to detect antibodies bound to NS1 protein. Abs. indicates the optical density at 650 nm. P1: peptide-1, P2: peptide-2, P3: peptide-3, P4: peptide-4, P5: peptide-5, P6: peptide-6, and P7: peptide-7. PC: positive control (run without added synthetic peptide)

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques: Competitive ELISA, Recombinant, Enzyme-linked Immunosorbent Assay, Membrane, Positive Control

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Detection of recombinant NS1 proteins of DENV-1 to − 4 by RDTs. Recombinant DENV NS1 proteins were serially diluted in PBS. The lower limits of detection for the RDTs were defined by chromatographing dilutions of the recombinant NS1 proteins and then quantifying the signal color intensities (mAbs: milli-absorbance units). Blue circles, red squares, and gray crosses indicate results of the TKK-1st kit, the TKK-2nd kit, and a SD Bioline kit, respectively

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques: Recombinant

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Detection of NS1 in culture supernatants of laboratory strains (DENV-1 to 4) by RDTs. Culture supernatants from Vero cells infected with each serotype of DENV (DENV-1: Mochizuki strain, DENV-2: 16681 strain, DENV-3: H87 strain, DENV-4: H241 strain) were chromatographed on each RDT. The vertical axis indicates intensity of color (mAbs) measured by an Immunochromato-Reader; the horizontal axis indicates virus concentration (RNA copies/mL) based on RT-PCR. Blue circles, red boxes, gray crosses, and yellow triangles indicate results of the TKK-1st kit, TKK-2nd kit, SD Bioline kit, and PanBio kit, respectively

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques: Infection, Virus, Concentration Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Detection of clinical DENV isolates from Bangladesh. Culture supernatants of Vero cells infected with clinical isolates (DENV-1: 1387 and 1547; DENV-2: 1431, 1482, 1556, and 1634; DENV-3: 1471 and 1479) isolated from serum collected in 2017, at Apollo Hospitals Dhaka, Bangladesh, were serially diluted, and applied to RDTs. The y-axis indicates intensity of color (mAbs) measured by an Immunochromato-Reader and the x-axis indicates virus concentration (RNA copies/mL) based on RT-PCR. Blue, red, and gray circles indicate results of the TKK-1st kit, TKK-2nd kit, and SD Bioline kit, respectively

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques: Infection, Isolation, Virus, Concentration Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Detection of NS1 protein by ELISA. a Serially diluted recombinant NS1 proteins were applied to ELISA. b Culture supernatants of Vero cells infected with the indicated virus strains were serially diluted and applied to ELISA. c Correlation between ELISA and RDTs. The x-axis indicates color intensities (mAbs: milli absorbance) of TKK-1st kit (blue circles) and TKK-2nd kit (red triangles) and the y-axis indicates optical densities at 450 nm of ELISA from 29 DENV-PCR positive samples

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Infection, Virus

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Amino acid variation in positions 100 to 122 of the NS1 protein. Blue color denotes the dengue virus (DENV) obtained in Bangladesh in 2017. a All the other DENV-1 sequences determined in the present study had the same sequence as S1387 in positions 100–122. b All the other DENV-2 sequences determined in the present study (except for S1482, S1431, and S1285) had the same sequence as S1489 in positions 100–122. c S1431 and S1285 had the same sequence as S1482 in positions 100–122. d All the other DENV-3 sequences determined in the present study had the same sequence as S1471 in positions 100–122.Yellow, orange, gray, and green cells indicate the DENV-1, DENV-2, DENV-3, and DENV-4-specific amino acids, respectively. Blue cells indicate amino acids that differ from those of DENV. Red colors denote amino acid variation within the serotype. ZIKA: Zika virus, MR766 strain, Q32ZE1 JEV: Japanese encephalitis virus, Nakayama strain, ABQ52691 WNV: West Nile virus, 2002 epidemic strain, AAV54504 YFV: yellow fever virus, French neurotropic strain, AAA99712 TBEV: tick-bone encephalitis virus, 263 strain, AAA86739

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques: Virus, Sequencing

Journal: Virology Journal

Article Title: Evaluation of novel rapid detection kits for dengue virus NS1 antigen in Dhaka, Bangladesh, in 2017

doi: 10.1186/s12985-019-1204-y

Figure Lengend Snippet: Correlation between color intensity of RDTs and Ct values of DENV detection PCR. Upper and right column bar graphs show distributions of color intensities and Ct values. A correlation coefficient and p value for each dot plot is shown in the upper area of each dot-plot graph. The left panel (blue color) is for the TKK-1st kit. The center panel (green color) is for the TKK-2nd kit. The right panel (red color) is for the SD Bioline kit

Article Snippet: Twelve mouse MAbs against DENV NS1 protein were purchased from Bio Matrix Research, Inc. Nagareyama, Japan (Catalog No. BMRdn).

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Label-Free Electrochemical Biosensors for the Determination of Flaviviruses : Dengue, Zika, and Japanese Encephalitis

doi: 10.3390/s20164600

Figure Lengend Snippet: Survey of electrochemical label-free biosensors for Zika diagnostic.

Article Snippet: Microtiter plate precoated with ZIKV NS1/ Euroimmun anti-ZIKV IgM , Indirect ELISA , Antibody-HRP conjugates , DENV Ig M antibodies , 56% , West Nile virus , d, f , a, c , [ , ] .

Techniques: Diagnostic Assay, Virus, Polymer, Membrane, Indirect ELISA

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: (a) Characteristics of all the included studies. (b) Specific ICT manufacturers, sensitivity, and specificity in included primary studies.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Isolation, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Indirect ELISA, HI Assay, Stripping Membranes, Diagnostic Assay

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: Summary forest plot for IgM (only), NS1 (only), and NS1/IgM tests in the acute phase [ , , , , , , , , , , , , , , ].

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: Summary findings of DOR when subgrouping ICTs: NS1 (only), IgM (only), NS1/IgM. Additionally, ELISA and RT-PCR were pooled to note reference test effects on DOR of DENV.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: NS1 (Only) summary findings of post-hoc analysis.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Isolation, Stripping Membranes, Diagnostic Assay, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Indirect ELISA

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: IgM (Only) Summary Findings of Post–Hoc Analysis.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Isolation, HI Assay

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: NS1/IgM Summary Findings of Post–Hoc Analysis.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Isolation, Stripping Membranes, Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Indirect ELISA

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: Summary ROC plot of tests: (1) IgM, (2) NS1, (3) NS1/IgM.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: