zikv ns1 specific antibody elisa Search Results


zikv ns1 monoclonal ab  (Native Antigen Inc)
94
Native Antigen Inc zikv ns1 monoclonal ab
Zikv Ns1 Monoclonal Ab, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ns1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology ns1
Ns1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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anti-denv ns1 type 1–4 elisa  (EUROIMMUN)
90
EUROIMMUN anti-denv ns1 type 1–4 elisa
Anti Denv Ns1 Type 1–4 Elisa, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hdac1  (Abcam)
99
Abcam hdac1
Hdac1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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shp 2  (Proteintech)
93
Proteintech shp 2
Inhibitory effect of CTS on p-STAT3 is mediated by <t>SHP-2.</t> ( a ) U251 cells were treated with pervanadate at indicated concentration for 1h, and with additional 10 μ M CTS for 2 h. Proteins were analyzed by western blotting with STAT3, p-STAT3 (Tyr705) and GAPDH antibodies. (PI: phosphoesterase inhibitors, pervanadate). ( b ) U251 cells were treated with 10 μ M CTS for 0, 0.15, 0.5, 1, 2, 4 h. Proteins were analyzed by western blotting with SHP-1, TC-PTP, SHP-2, p-SHP-2 (Tyr542), p-SHP-2 (Tyr580) and GAPDH antibodies. ( c – e ) U251 cells were transfected with specific phosphates siRNA including SHP-1 ( c ), TC-PTP ( d ), SHP-2 ( e ) or negative control (NC) for 48 h, and then treated with CTS with the indicated concentration for 120 min, followed by western blot analysis with the indicated phosphates antibodies. Expression of p-STAT3 (Tyr705) relative to STAT3 were quantified by densitometry. Results are the mean±S.E.M. ( n =3 for each group). ## P <0.01, ### P <0.001 versus control. ( f ) Cell lysates from U251 cells treated with 0, 5, 10 μ M CTS for 2 h were subjected to SHP-2 phosphatase activity assay. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01 versus CTS 0 μ M group. ( g and h ) U251 cells were pretreated with 25 μ M NSC-87877 for 30 min, followed by 10 μ M CTS treatment for 120 min. Total cell lysates were prepared and subjected to western blot analysis with indicated antibodies ( g ) or SHP-2 phosphatase activity assay ( h ). Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus CTS 0 μ M group
Shp 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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anti dna pkcs phospho ser2056  (Abcam)
99
Abcam anti dna pkcs phospho ser2056
Inhibitory effect of CTS on p-STAT3 is mediated by <t>SHP-2.</t> ( a ) U251 cells were treated with pervanadate at indicated concentration for 1h, and with additional 10 μ M CTS for 2 h. Proteins were analyzed by western blotting with STAT3, p-STAT3 (Tyr705) and GAPDH antibodies. (PI: phosphoesterase inhibitors, pervanadate). ( b ) U251 cells were treated with 10 μ M CTS for 0, 0.15, 0.5, 1, 2, 4 h. Proteins were analyzed by western blotting with SHP-1, TC-PTP, SHP-2, p-SHP-2 (Tyr542), p-SHP-2 (Tyr580) and GAPDH antibodies. ( c – e ) U251 cells were transfected with specific phosphates siRNA including SHP-1 ( c ), TC-PTP ( d ), SHP-2 ( e ) or negative control (NC) for 48 h, and then treated with CTS with the indicated concentration for 120 min, followed by western blot analysis with the indicated phosphates antibodies. Expression of p-STAT3 (Tyr705) relative to STAT3 were quantified by densitometry. Results are the mean±S.E.M. ( n =3 for each group). ## P <0.01, ### P <0.001 versus control. ( f ) Cell lysates from U251 cells treated with 0, 5, 10 μ M CTS for 2 h were subjected to SHP-2 phosphatase activity assay. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01 versus CTS 0 μ M group. ( g and h ) U251 cells were pretreated with 25 μ M NSC-87877 for 30 min, followed by 10 μ M CTS treatment for 120 min. Total cell lysates were prepared and subjected to western blot analysis with indicated antibodies ( g ) or SHP-2 phosphatase activity assay ( h ). Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus CTS 0 μ M group
Anti Dna Pkcs Phospho Ser2056, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-zikv ns-1 and capsid protein polyclonal antibodies  (GeneTex)
90
GeneTex rabbit anti-zikv ns-1 and capsid protein polyclonal antibodies
Inhibitory effect of CTS on p-STAT3 is mediated by <t>SHP-2.</t> ( a ) U251 cells were treated with pervanadate at indicated concentration for 1h, and with additional 10 μ M CTS for 2 h. Proteins were analyzed by western blotting with STAT3, p-STAT3 (Tyr705) and GAPDH antibodies. (PI: phosphoesterase inhibitors, pervanadate). ( b ) U251 cells were treated with 10 μ M CTS for 0, 0.15, 0.5, 1, 2, 4 h. Proteins were analyzed by western blotting with SHP-1, TC-PTP, SHP-2, p-SHP-2 (Tyr542), p-SHP-2 (Tyr580) and GAPDH antibodies. ( c – e ) U251 cells were transfected with specific phosphates siRNA including SHP-1 ( c ), TC-PTP ( d ), SHP-2 ( e ) or negative control (NC) for 48 h, and then treated with CTS with the indicated concentration for 120 min, followed by western blot analysis with the indicated phosphates antibodies. Expression of p-STAT3 (Tyr705) relative to STAT3 were quantified by densitometry. Results are the mean±S.E.M. ( n =3 for each group). ## P <0.01, ### P <0.001 versus control. ( f ) Cell lysates from U251 cells treated with 0, 5, 10 μ M CTS for 2 h were subjected to SHP-2 phosphatase activity assay. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01 versus CTS 0 μ M group. ( g and h ) U251 cells were pretreated with 25 μ M NSC-87877 for 30 min, followed by 10 μ M CTS treatment for 120 min. Total cell lysates were prepared and subjected to western blot analysis with indicated antibodies ( g ) or SHP-2 phosphatase activity assay ( h ). Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus CTS 0 μ M group
Rabbit Anti Zikv Ns 1 And Capsid Protein Polyclonal Antibodies, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-aiv ns1  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology mouse anti-aiv ns1
Inhibitory effect of CTS on p-STAT3 is mediated by <t>SHP-2.</t> ( a ) U251 cells were treated with pervanadate at indicated concentration for 1h, and with additional 10 μ M CTS for 2 h. Proteins were analyzed by western blotting with STAT3, p-STAT3 (Tyr705) and GAPDH antibodies. (PI: phosphoesterase inhibitors, pervanadate). ( b ) U251 cells were treated with 10 μ M CTS for 0, 0.15, 0.5, 1, 2, 4 h. Proteins were analyzed by western blotting with SHP-1, TC-PTP, SHP-2, p-SHP-2 (Tyr542), p-SHP-2 (Tyr580) and GAPDH antibodies. ( c – e ) U251 cells were transfected with specific phosphates siRNA including SHP-1 ( c ), TC-PTP ( d ), SHP-2 ( e ) or negative control (NC) for 48 h, and then treated with CTS with the indicated concentration for 120 min, followed by western blot analysis with the indicated phosphates antibodies. Expression of p-STAT3 (Tyr705) relative to STAT3 were quantified by densitometry. Results are the mean±S.E.M. ( n =3 for each group). ## P <0.01, ### P <0.001 versus control. ( f ) Cell lysates from U251 cells treated with 0, 5, 10 μ M CTS for 2 h were subjected to SHP-2 phosphatase activity assay. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01 versus CTS 0 μ M group. ( g and h ) U251 cells were pretreated with 25 μ M NSC-87877 for 30 min, followed by 10 μ M CTS treatment for 120 min. Total cell lysates were prepared and subjected to western blot analysis with indicated antibodies ( g ) or SHP-2 phosphatase activity assay ( h ). Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus CTS 0 μ M group
Mouse Anti Aiv Ns1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-jev ns1 polyclonal antibody gtx131370  (GeneTex)
90
GeneTex rabbit anti-jev ns1 polyclonal antibody gtx131370
Analysis of stability of <t>JEV-HA/NS2A/∆NS1’.</t> The full-length gene sequence of JEV-HA/NS2A/∆NS1’ was amplified by RT-PCR from passage 6 and subjected to DNA sequencing. ( A ) Sequencing chromatogram of HA-tag insertion. ( B ) Sequencing chromatogram of A30P mutation. ( C , D ) BHK-21 cells were infected with the indicated recombinant JEV and subjected to western blots ( C ) and IFA analysis ( D ). Detection of HA-NS2A expression by IFA. HA-NS2A (green) and NS3 (red) were detected with anti-HA and anti-NS3 antibodies, respectively. The nuclei (blue) were stained with 4’,6-diamidino-2-phenylindole (DAPI).
Rabbit Anti Jev Ns1 Polyclonal Antibody Gtx131370, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zikv ns1 protein antibody  (Thermo Fisher)
90
Thermo Fisher zikv ns1 protein antibody
The blood-fed infection of <t>ZIKV</t> or DENV-2 in Ar. subalbatus . A Schematic diagram of oral infection experiment. B Infection rates and ( C ) RNA copies of ZIKV in various tissues at different days post inoculation (dpi). The results are present as the means ± SD . Error bars indicate SD s. The experiment was repeated three times. SD : Standard deviation.
Zikv Ns1 Protein Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sd ns1 igm  (Bio-Rad)
93
Bio-Rad sd ns1 igm
(a) Characteristics of all the included studies. (b) Specific ICT manufacturers, sensitivity, and specificity in included primary studies.
Sd Ns1 Igm, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Inhibitory effect of CTS on p-STAT3 is mediated by SHP-2. ( a ) U251 cells were treated with pervanadate at indicated concentration for 1h, and with additional 10 μ M CTS for 2 h. Proteins were analyzed by western blotting with STAT3, p-STAT3 (Tyr705) and GAPDH antibodies. (PI: phosphoesterase inhibitors, pervanadate). ( b ) U251 cells were treated with 10 μ M CTS for 0, 0.15, 0.5, 1, 2, 4 h. Proteins were analyzed by western blotting with SHP-1, TC-PTP, SHP-2, p-SHP-2 (Tyr542), p-SHP-2 (Tyr580) and GAPDH antibodies. ( c – e ) U251 cells were transfected with specific phosphates siRNA including SHP-1 ( c ), TC-PTP ( d ), SHP-2 ( e ) or negative control (NC) for 48 h, and then treated with CTS with the indicated concentration for 120 min, followed by western blot analysis with the indicated phosphates antibodies. Expression of p-STAT3 (Tyr705) relative to STAT3 were quantified by densitometry. Results are the mean±S.E.M. ( n =3 for each group). ## P <0.01, ### P <0.001 versus control. ( f ) Cell lysates from U251 cells treated with 0, 5, 10 μ M CTS for 2 h were subjected to SHP-2 phosphatase activity assay. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01 versus CTS 0 μ M group. ( g and h ) U251 cells were pretreated with 25 μ M NSC-87877 for 30 min, followed by 10 μ M CTS treatment for 120 min. Total cell lysates were prepared and subjected to western blot analysis with indicated antibodies ( g ) or SHP-2 phosphatase activity assay ( h ). Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus CTS 0 μ M group

Journal: Cell Death & Disease

Article Title: Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation

doi: 10.1038/cddis.2017.174

Figure Lengend Snippet: Inhibitory effect of CTS on p-STAT3 is mediated by SHP-2. ( a ) U251 cells were treated with pervanadate at indicated concentration for 1h, and with additional 10 μ M CTS for 2 h. Proteins were analyzed by western blotting with STAT3, p-STAT3 (Tyr705) and GAPDH antibodies. (PI: phosphoesterase inhibitors, pervanadate). ( b ) U251 cells were treated with 10 μ M CTS for 0, 0.15, 0.5, 1, 2, 4 h. Proteins were analyzed by western blotting with SHP-1, TC-PTP, SHP-2, p-SHP-2 (Tyr542), p-SHP-2 (Tyr580) and GAPDH antibodies. ( c – e ) U251 cells were transfected with specific phosphates siRNA including SHP-1 ( c ), TC-PTP ( d ), SHP-2 ( e ) or negative control (NC) for 48 h, and then treated with CTS with the indicated concentration for 120 min, followed by western blot analysis with the indicated phosphates antibodies. Expression of p-STAT3 (Tyr705) relative to STAT3 were quantified by densitometry. Results are the mean±S.E.M. ( n =3 for each group). ## P <0.01, ### P <0.001 versus control. ( f ) Cell lysates from U251 cells treated with 0, 5, 10 μ M CTS for 2 h were subjected to SHP-2 phosphatase activity assay. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01 versus CTS 0 μ M group. ( g and h ) U251 cells were pretreated with 25 μ M NSC-87877 for 30 min, followed by 10 μ M CTS treatment for 120 min. Total cell lysates were prepared and subjected to western blot analysis with indicated antibodies ( g ) or SHP-2 phosphatase activity assay ( h ). Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus CTS 0 μ M group

Article Snippet: Primary antibodies were used as follows: antibodies against SHP-1 and SHP-2 (Epitomics, Burlingame, CA, USA); STAT3, p-STAT3 (Tyr705), p-STAT3 (Ser727), p-p44/42 Erk1/2 (Thr202/Tyr204), cyclin D1, cyclin E1, survivin, p-SHP-2 (Tyr580), p-SHP-2 (Tyr542) and Histon H3 (Cell Signaling Technology); antibody against Ki67 (BD Bioscience); antibodies against TC-PTP, SHP-2, Mcl-1 (Proteintech, Wuhan, Hubei, China); GAPDH (Beyotime).

Techniques: Concentration Assay, Western Blot, Transfection, Negative Control, Expressing, Phosphatase Assay

Growth inhibition of intracranial tumor by CTS treatment extended survival of nude mice bearing intracerebral U87 xenografts treated with CTS. ( a ) Growth inhibition of intracranial tumor by CTS treatment. Left: after hematoxylin–eosin staining, tumor size was determined by the areas that were measured at the maximal brain tumor dimensions in the coronal sections. The red circles indicate tumor tissue. Scale bar, 1 mm. Data were calculated by taking the tumor size of control as 100%. Data are expressed as mean±S.E.M., n =5 for each group. * P <0.05; ** P <0.01 versus Model control group. Right: higher magnification in HE staining. Scale bar, 50 μ m. ( b ) After xenografts for 7 days, nude mice were treated with CTS from day 8 to 21 and were observed after discontinuation of therapy. Statistical significance was achieved by Cox–Mantel and Wilcoxon analyses of a Kaplan–Meier survival curve ( n =5). ( c ) Some samples from the harvested tumor tissue on Day 28 were used in detecting tyrosine phosphatase activity of SHP-2. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus Model control group

Journal: Cell Death & Disease

Article Title: Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation

doi: 10.1038/cddis.2017.174

Figure Lengend Snippet: Growth inhibition of intracranial tumor by CTS treatment extended survival of nude mice bearing intracerebral U87 xenografts treated with CTS. ( a ) Growth inhibition of intracranial tumor by CTS treatment. Left: after hematoxylin–eosin staining, tumor size was determined by the areas that were measured at the maximal brain tumor dimensions in the coronal sections. The red circles indicate tumor tissue. Scale bar, 1 mm. Data were calculated by taking the tumor size of control as 100%. Data are expressed as mean±S.E.M., n =5 for each group. * P <0.05; ** P <0.01 versus Model control group. Right: higher magnification in HE staining. Scale bar, 50 μ m. ( b ) After xenografts for 7 days, nude mice were treated with CTS from day 8 to 21 and were observed after discontinuation of therapy. Statistical significance was achieved by Cox–Mantel and Wilcoxon analyses of a Kaplan–Meier survival curve ( n =5). ( c ) Some samples from the harvested tumor tissue on Day 28 were used in detecting tyrosine phosphatase activity of SHP-2. Data are expressed as mean±S.E.M., n =3 for each group. * P <0.05; ** P <0.01, versus Model control group

Article Snippet: Primary antibodies were used as follows: antibodies against SHP-1 and SHP-2 (Epitomics, Burlingame, CA, USA); STAT3, p-STAT3 (Tyr705), p-STAT3 (Ser727), p-p44/42 Erk1/2 (Thr202/Tyr204), cyclin D1, cyclin E1, survivin, p-SHP-2 (Tyr580), p-SHP-2 (Tyr542) and Histon H3 (Cell Signaling Technology); antibody against Ki67 (BD Bioscience); antibodies against TC-PTP, SHP-2, Mcl-1 (Proteintech, Wuhan, Hubei, China); GAPDH (Beyotime).

Techniques: Inhibition, Staining, Activity Assay

Analysis of stability of JEV-HA/NS2A/∆NS1’. The full-length gene sequence of JEV-HA/NS2A/∆NS1’ was amplified by RT-PCR from passage 6 and subjected to DNA sequencing. ( A ) Sequencing chromatogram of HA-tag insertion. ( B ) Sequencing chromatogram of A30P mutation. ( C , D ) BHK-21 cells were infected with the indicated recombinant JEV and subjected to western blots ( C ) and IFA analysis ( D ). Detection of HA-NS2A expression by IFA. HA-NS2A (green) and NS3 (red) were detected with anti-HA and anti-NS3 antibodies, respectively. The nuclei (blue) were stained with 4’,6-diamidino-2-phenylindole (DAPI).

Journal: Viruses

Article Title: Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection

doi: 10.3390/v14040706

Figure Lengend Snippet: Analysis of stability of JEV-HA/NS2A/∆NS1’. The full-length gene sequence of JEV-HA/NS2A/∆NS1’ was amplified by RT-PCR from passage 6 and subjected to DNA sequencing. ( A ) Sequencing chromatogram of HA-tag insertion. ( B ) Sequencing chromatogram of A30P mutation. ( C , D ) BHK-21 cells were infected with the indicated recombinant JEV and subjected to western blots ( C ) and IFA analysis ( D ). Detection of HA-NS2A expression by IFA. HA-NS2A (green) and NS3 (red) were detected with anti-HA and anti-NS3 antibodies, respectively. The nuclei (blue) were stained with 4’,6-diamidino-2-phenylindole (DAPI).

Article Snippet: The commercial antibodies used in this study consisted of: mouse anti-HA monoclonal antibody (H9658, Sigma, St. Louis, MO, USA); rabbit anti-calnexin polyclonal antibody (C4731, Sigma); rabbit anti-Erlin-2 antibody (EPR8088, Abcam, Shanghai, China); mouse anti-β-actin monoclonal antibody (Proteintech Group, Chicago, IL, USA); rabbit anti-JEV C polyclonal antibody (GTX131368, GeneTex, St. Anthony, TX, USA); rabbit anti-JEV E polyclonal antibody (GTX125867, GeneTex); rabbit anti-JEV NS1 polyclonal antibody (GTX131370, GeneTex); mouse anti-JEV NS1 monoclonal antibody (GTX633820, GeneTex); rabbit anti-JEV NS2B polyclonal antibody (GTX125972, GeneTex); rabbit anti-JEV NS4A polyclonal antibody (GTX132028, GeneTex); and rabbit anti-JEV NS4B polyclonal antibody (GTX125865, GeneTex).

Techniques: Sequencing, Amplification, Reverse Transcription Polymerase Chain Reaction, DNA Sequencing, Mutagenesis, Infection, Recombinant, Western Blot, Expressing, Staining

Replication of JEV-HA/NS2A/∆NS1’ in BHK-21 cells. ( A , B ) BHK-21 cells were infected with the indicated viruses at an MOI of 0.01. Viral titers in the supernatants and viral protein expression in the cells were examined at the indicated time points by TCID 50 assays ( A ) and western blot ( B ), respectively. ( C , D ) Plaque morphology of recombinant JEV. BHK-21 cell monolayers were infected with the indicated viruses for an analysis of plaque morphology. The plaques were stained with crystal violet at 5 dpi ( C ) and the plaque diameters were measured and plotted ( D ). The significant differences between the groups were tested by Student’s t -test.

Journal: Viruses

Article Title: Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection

doi: 10.3390/v14040706

Figure Lengend Snippet: Replication of JEV-HA/NS2A/∆NS1’ in BHK-21 cells. ( A , B ) BHK-21 cells were infected with the indicated viruses at an MOI of 0.01. Viral titers in the supernatants and viral protein expression in the cells were examined at the indicated time points by TCID 50 assays ( A ) and western blot ( B ), respectively. ( C , D ) Plaque morphology of recombinant JEV. BHK-21 cell monolayers were infected with the indicated viruses for an analysis of plaque morphology. The plaques were stained with crystal violet at 5 dpi ( C ) and the plaque diameters were measured and plotted ( D ). The significant differences between the groups were tested by Student’s t -test.

Article Snippet: The commercial antibodies used in this study consisted of: mouse anti-HA monoclonal antibody (H9658, Sigma, St. Louis, MO, USA); rabbit anti-calnexin polyclonal antibody (C4731, Sigma); rabbit anti-Erlin-2 antibody (EPR8088, Abcam, Shanghai, China); mouse anti-β-actin monoclonal antibody (Proteintech Group, Chicago, IL, USA); rabbit anti-JEV C polyclonal antibody (GTX131368, GeneTex, St. Anthony, TX, USA); rabbit anti-JEV E polyclonal antibody (GTX125867, GeneTex); rabbit anti-JEV NS1 polyclonal antibody (GTX131370, GeneTex); mouse anti-JEV NS1 monoclonal antibody (GTX633820, GeneTex); rabbit anti-JEV NS2B polyclonal antibody (GTX125972, GeneTex); rabbit anti-JEV NS4A polyclonal antibody (GTX132028, GeneTex); and rabbit anti-JEV NS4B polyclonal antibody (GTX125865, GeneTex).

Techniques: Infection, Expressing, Western Blot, Recombinant, Staining

HA-NS2A localization to the ER. ( A ) BHK-21 cells were infected with JEV-HA/NS2A/∆NS1’ and harvested at 48 hpi for an isolation of the detergent-resistant membranes (DRM). The presence of the indicated proteins in the DRM was detected by western blots. ( B ) BHK-21 cells were infected with JEV-HA/NS2A/∆NS1’ and subjected to an immunofluorescence assay at 24 hpi. HA-NS2A (green) and the ER (red) were detected with anti-HA and anti-calnexin antibodies, respectively. The nuclei (blue) were stained with DAPI.

Journal: Viruses

Article Title: Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection

doi: 10.3390/v14040706

Figure Lengend Snippet: HA-NS2A localization to the ER. ( A ) BHK-21 cells were infected with JEV-HA/NS2A/∆NS1’ and harvested at 48 hpi for an isolation of the detergent-resistant membranes (DRM). The presence of the indicated proteins in the DRM was detected by western blots. ( B ) BHK-21 cells were infected with JEV-HA/NS2A/∆NS1’ and subjected to an immunofluorescence assay at 24 hpi. HA-NS2A (green) and the ER (red) were detected with anti-HA and anti-calnexin antibodies, respectively. The nuclei (blue) were stained with DAPI.

Article Snippet: The commercial antibodies used in this study consisted of: mouse anti-HA monoclonal antibody (H9658, Sigma, St. Louis, MO, USA); rabbit anti-calnexin polyclonal antibody (C4731, Sigma); rabbit anti-Erlin-2 antibody (EPR8088, Abcam, Shanghai, China); mouse anti-β-actin monoclonal antibody (Proteintech Group, Chicago, IL, USA); rabbit anti-JEV C polyclonal antibody (GTX131368, GeneTex, St. Anthony, TX, USA); rabbit anti-JEV E polyclonal antibody (GTX125867, GeneTex); rabbit anti-JEV NS1 polyclonal antibody (GTX131370, GeneTex); mouse anti-JEV NS1 monoclonal antibody (GTX633820, GeneTex); rabbit anti-JEV NS2B polyclonal antibody (GTX125972, GeneTex); rabbit anti-JEV NS4A polyclonal antibody (GTX132028, GeneTex); and rabbit anti-JEV NS4B polyclonal antibody (GTX125865, GeneTex).

Techniques: Infection, Isolation, Western Blot, Immunofluorescence, Staining

Interaction of HA-NS2A with viral proteins. BHK-21 cells were infected with JEV and harvested at 48 hpi for a co-immunoprecipitation assay. ( A ) The cell lysates were incubated with anti-HA antibodies and the immunoprecipitated proteins were detected with the indicated antibodies against different viral proteins. ( B ) The cell lysates were pre-treated with RNase A and the presence of NS1 and GAPDH RNAs was examined by RT-PCR. ( C ) The cells lysates were pre-treated with RNase A and subsequently subjected to co-immunoprecipitation with anti-HA antibodies. The immunoprecipitated proteins were detected by the indicated antibodies against different viral proteins.

Journal: Viruses

Article Title: Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection

doi: 10.3390/v14040706

Figure Lengend Snippet: Interaction of HA-NS2A with viral proteins. BHK-21 cells were infected with JEV and harvested at 48 hpi for a co-immunoprecipitation assay. ( A ) The cell lysates were incubated with anti-HA antibodies and the immunoprecipitated proteins were detected with the indicated antibodies against different viral proteins. ( B ) The cell lysates were pre-treated with RNase A and the presence of NS1 and GAPDH RNAs was examined by RT-PCR. ( C ) The cells lysates were pre-treated with RNase A and subsequently subjected to co-immunoprecipitation with anti-HA antibodies. The immunoprecipitated proteins were detected by the indicated antibodies against different viral proteins.

Article Snippet: The commercial antibodies used in this study consisted of: mouse anti-HA monoclonal antibody (H9658, Sigma, St. Louis, MO, USA); rabbit anti-calnexin polyclonal antibody (C4731, Sigma); rabbit anti-Erlin-2 antibody (EPR8088, Abcam, Shanghai, China); mouse anti-β-actin monoclonal antibody (Proteintech Group, Chicago, IL, USA); rabbit anti-JEV C polyclonal antibody (GTX131368, GeneTex, St. Anthony, TX, USA); rabbit anti-JEV E polyclonal antibody (GTX125867, GeneTex); rabbit anti-JEV NS1 polyclonal antibody (GTX131370, GeneTex); mouse anti-JEV NS1 monoclonal antibody (GTX633820, GeneTex); rabbit anti-JEV NS2B polyclonal antibody (GTX125972, GeneTex); rabbit anti-JEV NS4A polyclonal antibody (GTX132028, GeneTex); and rabbit anti-JEV NS4B polyclonal antibody (GTX125865, GeneTex).

Techniques: Infection, Co-Immunoprecipitation Assay, Incubation, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction

The blood-fed infection of ZIKV or DENV-2 in Ar. subalbatus . A Schematic diagram of oral infection experiment. B Infection rates and ( C ) RNA copies of ZIKV in various tissues at different days post inoculation (dpi). The results are present as the means ± SD . Error bars indicate SD s. The experiment was repeated three times. SD : Standard deviation.

Journal: Infectious Diseases of Poverty

Article Title: Armigeres subalbatus is a potential vector for Zika virus but not dengue virus

doi: 10.1186/s40249-022-00990-0

Figure Lengend Snippet: The blood-fed infection of ZIKV or DENV-2 in Ar. subalbatus . A Schematic diagram of oral infection experiment. B Infection rates and ( C ) RNA copies of ZIKV in various tissues at different days post inoculation (dpi). The results are present as the means ± SD . Error bars indicate SD s. The experiment was repeated three times. SD : Standard deviation.

Article Snippet: F Virus particles were detected with ZIKV NS1 protein antibody (Thermo Fisher) by IHC and are displayed as obvious brownish-red marked by red arrow The detection result of fourth instar larvae (41 pools) showed that the larvae of Ar. subalbatus could not be infected by DENV-2 in the continuous addition experiment. (Additional file : Table S3).

Techniques: Infection, Standard Deviation

The artificial urine infection of ZIKV or DENV-2 in the larvae of Ar. subalbatus . A Schematic diagram of the artificial virus urine infection experiment. B Susceptibility of different instar larvae reared in artificial urine contained ZIKV, and virus was detected in the fourth instar larvae. C RNA copies of ZIKV in infected midguts, ovaries, and salivary glands of adults. The results are expressed as the means ± standard errors (SEs). D The third instar larvae were reared in artificial urine with ZIKV, and the infection of larvae was detected at 1, 2, 3, 4 days post inoculation (dpi) with RT-PCR and RT-qPCR. E ZIKV was detected in midgut, anal papillae and carcass of fourth instar larvae at 4dpi with RT-PCR and RT-qPCR. F Virus particles were detected with ZIKV NS1 protein antibody (Thermo Fisher) by IHC and are displayed as obvious brownish-red marked by red arrow

Journal: Infectious Diseases of Poverty

Article Title: Armigeres subalbatus is a potential vector for Zika virus but not dengue virus

doi: 10.1186/s40249-022-00990-0

Figure Lengend Snippet: The artificial urine infection of ZIKV or DENV-2 in the larvae of Ar. subalbatus . A Schematic diagram of the artificial virus urine infection experiment. B Susceptibility of different instar larvae reared in artificial urine contained ZIKV, and virus was detected in the fourth instar larvae. C RNA copies of ZIKV in infected midguts, ovaries, and salivary glands of adults. The results are expressed as the means ± standard errors (SEs). D The third instar larvae were reared in artificial urine with ZIKV, and the infection of larvae was detected at 1, 2, 3, 4 days post inoculation (dpi) with RT-PCR and RT-qPCR. E ZIKV was detected in midgut, anal papillae and carcass of fourth instar larvae at 4dpi with RT-PCR and RT-qPCR. F Virus particles were detected with ZIKV NS1 protein antibody (Thermo Fisher) by IHC and are displayed as obvious brownish-red marked by red arrow

Article Snippet: F Virus particles were detected with ZIKV NS1 protein antibody (Thermo Fisher) by IHC and are displayed as obvious brownish-red marked by red arrow The detection result of fourth instar larvae (41 pools) showed that the larvae of Ar. subalbatus could not be infected by DENV-2 in the continuous addition experiment. (Additional file : Table S3).

Techniques: Infection, Virus, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

Infection rate of larvae for ZIKV in artificial urine

Journal: Infectious Diseases of Poverty

Article Title: Armigeres subalbatus is a potential vector for Zika virus but not dengue virus

doi: 10.1186/s40249-022-00990-0

Figure Lengend Snippet: Infection rate of larvae for ZIKV in artificial urine

Article Snippet: F Virus particles were detected with ZIKV NS1 protein antibody (Thermo Fisher) by IHC and are displayed as obvious brownish-red marked by red arrow The detection result of fourth instar larvae (41 pools) showed that the larvae of Ar. subalbatus could not be infected by DENV-2 in the continuous addition experiment. (Additional file : Table S3).

Techniques: Infection

(a) Characteristics of all the included studies. (b) Specific ICT manufacturers, sensitivity, and specificity in included primary studies.

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: (a) Characteristics of all the included studies. (b) Specific ICT manufacturers, sensitivity, and specificity in included primary studies.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Isolation, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Indirect ELISA, HI Assay, Stripping Membranes, Diagnostic Assay

NS1/IgM Summary Findings of Post–Hoc Analysis.

Journal: International Journal of Environmental Research and Public Health

Article Title: Diagnostic Accuracy of Various Immunochromatographic Tests for NS1 Antigen and IgM Antibodies Detection in Acute Dengue Virus Infection

doi: 10.3390/ijerph19148756

Figure Lengend Snippet: NS1/IgM Summary Findings of Post–Hoc Analysis.

Article Snippet: 15 , Tricou, 2010, Vietnam [ ] , Bio-Rad NS1 Ag Strip and SD Dengue Duo (NS1/IgM/IgG) lateral flow rapid tests , , NS1 Bio-Rad: 61.6 (55.2–67.8); NS1 SD: 62.4 (56.1–68.5) , 100% , NS1/IgM Bio–Rad: 83.3% (72.1–91.4); NS1/IgM/IgG Bio–Rad: 61.6%; NS1/IgM SD Duo: 75.5% (69.6–80.8); NS1/IgM/IgG SD Duo: 83.7% (78.4–88.1) , 100% , Primary: NS1 Biorad 80.3% (68.7–89.1), SD NS1 80.3% (68.7–89.1), SD NS1/IgM 83.3% (72.1–91.4), SD NS1/IgM/IgG 83.3% (72.1–91.4), Secondary: NS1 Biorad 55.1% (47.4–62.6), SD NS1 56.3% (48.6–63.7), SD NS1/IgM 72.7% (65.5–79.2), SD NS1/IgM/IgG 84.1% (77.8–89.2) , .

Techniques: Isolation, Stripping Membranes, Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Indirect ELISA